Avoiding, detecting, and inhibiting RNase
RNases, which play important roles in nucleic acid metabolism, are found in both prokaryotes and eukaryotes, and in practically every cell type. The human body uses RNases to defend against invading microorganisms by secreting these enzymes in fluids such as tears, saliva, mucus, and perspiration. RNases are also present in flaked skin, on hair that may fall onto a bench, and in pet hair that may cling to clothing. The primary source of RNases within most environments, however, is microorganisms—namely, bacteria and fungi. In this article, we discuss how to avoid, detect, and inhibit RNase when working with RNA.